px459 plasmid background Search Results


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Jackson Laboratory cre recombinase-encoding plasmid px459_pgk_cre

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Image Search Results


Journal: Cell Reports

Article Title: Xist-mediated silencing requires additive functions of SPEN and Polycomb together with differentiation-dependent recruitment of SmcHD1

doi: 10.1016/j.celrep.2022.110830

Figure Lengend Snippet:

Article Snippet: Cloning into sgRNA plasmid vectors was performed using reverse complement oligos and the single-step digestion-ligation Zhang lab protocol (Broad Institute) into the pX459 background ( ; Addgene plasmid #62988).

Techniques: Virus, Recombinant, Nick Translation, Clone Assay, CRISPR, Biomarker Discovery, Mutagenesis, Software